Overview
The T800-FLLED upright fluorescence microscope uses a cutting-edge 3-LED illuminator for variable-band excitation. Oufitted with the widely-used green (G1), blue (B1), and ultraviolet (UV2) excitation-bands, it can be used for a wide variety of common labeling or autofluorescence. Unlike single-LED fluorescence illuminators, the three LEDs are cycled using a multi-filter turret, eliminating the need to exchange light-sources. Each LED provides a precise excitation-source to eliminate non-relevant light, as well as providing maximum intensity at the targeted wavelength. Another major benefit of LED illumination is the elimination of long warm-up times and other care required by fluorescent light-sources such as mercury-vapor.
The infinity-corrected optical system includes plan-fluor objectives, which provide edge-to-edge sharpness with higher resolution, improved color-correction, and improved short and long wavelength transmission. The reversed quintuple-nosepiece is equipped with 4X, 10X, 20X, 40X, and 100X plan semi-apochromatic lenses. The 10X plan, high-eyepoint eyepieces provide a compounded magnification range of 40X to 1000X. The Koehler illumination-system is designed for precision, with a variable-NA swing-condenser allowing the use of high and low magnifications with full, even coverage. The robust frame offers excellent stability and ergonomics, with coaxial coarse and fine focusing, as well as coaxial stage-translation knobs providing fluid control.
Fluorescence
| Filters |
|
Filter Set |
Chart |
Excitation Fiter |
Dichromatic Mirror |
Emission Fiter |
Common Stains |
|
UV2 |
|
340-390nm |
400nm |
420nm |
Alexa Fluor (350, 405), BFP, DAPI, Dopamine, Hoechst (33258, 33342, 34580) |
|
B1 |
|
450-490nm |
495nm |
500-550nm |
Acridine Orange (DNA/RNA), Alexa Fluor (488, 500, 514, 610, 647), Chlorophyll, Cy2, DiO, GFP, Rhodamine (110, 123) |
|
G1 |
|
540-580nm |
585nm |
590-665nm |
Texas Red®, Texas Red® X, Cy3.5, Mito Tracker® Red |
|
| LED Excitation |
| Band |
Peak Wavelength |
| UV2 |
385nm |
| B1 |
470nm |
| G1 |
560nm |
|
Specifications
| Optical System |
Infinity-corrected |
| Head |
Trinocular, 30 ° incline, 360 ° rotatable |
| Interpupillary Adjustment |
Compensation-free gemel, 48-75mm |
| Eyepieces |
Plan 10X, high-eyepoint, FN 22mm, 30mm mount |
| Photo-port |
1X focus-adjustable C-mount |
| Nosepiece |
Quintuple reversed |
| Objective Lenses |
4X, 10X, 20X, 40X (spring), 100X (spring, oil) |
| Focusing System |
Coaxial coarse and fine focus, upper limit-stop, tension-control |
| Focus Travel |
30mm, 0.002mm fine-adjustment |
| Stage |
Double-layer mechanical 185mm x 165mm |
| X-Y Translation Range |
80mm x 55mm |
| Sub-stage Condenser-holder |
Rack-and-pinion, centerable |
| Condenser |
Achromatic swing condenser, NA1.2/0.22 |
| Transmitted Illumination |
3W LED, 6V2A, continous adjustment |
| Fluorescence Excitation Illumination |
3 x 3W LED: UV2(385nm), B1(470nm), G1(560nm) |
Objective Lenses
| Magnification |
Corrections |
NA |
Working Distance |
Cover-glass Thickness |
| 4X |
Plan Fluor |
0.13 |
15.13mm |
0.17mm |
| 10X |
Plan Fluor |
0.30 |
8.53mm |
0.17mm |
| 20X |
Plan Fluor |
0.50 |
2.33mm |
0.17mm |
| 40X |
Plan Fluor |
0.75 |
0.55mm |
0.17mm |
| 100X |
Plan Fluor |
1.28 |
0.21mm |
0.17mm |
Components
| Objectives |
| Plan Achromatic Objectives |
○ |
| Plan Fluor Objectives |
• |
| Plan Apochromatic Objectives |
○ |
|
| Eyepieces |
| 10X FN22 |
• |
| 15X |
○ |
| 20X |
○ |
| 25X |
○ |
|
| Condensers |
| Brightfield Swing Condenser (NA1.2/0.22) |
• |
| Dry Darkfield Condenser (NA0.90) |
○ |
| Oil Darkfield Condenser (NA1.25) |
○ |
| Phase-contrast Turret |
○ |
|
| Heads |
| Binocular Head |
○ |
| Trinocular Head |
• |
| Tilting Erect-image Trinocular Head |
○ |
|
Photo-ports
(Trinocular Heads Only) |
| 1X C-mount Adjustable Photo-port |
• |
| 0.5X C-mount Adjustable Photo-port |
○ |
| 0.3X C-mount Adjustable Photo-port |
○ |
| 23mm Adjustable Photo-port |
○ |
|
| • included |
○ optional |
– not available |
|
